01653nas a2200193   4500008004100000245009200041210006900133260000800202300001400210490000800224520099800232653001901230653001601249100001501265700002001280700001401300700001801314856012701332       2010                        eng d00aA low shear stress modular bioreactor for connected cell culture under high flow rates.0 alow shear stress modular bioreactor for connected cell culture u  cMay  a127–1370 v1063 a<p>A generic "system on a plate" modular multicompartmental bioreactor array which enables microwell protocols to be transferred directly to the bioreactor modules, without redesign of cell culture experiments or protocols is described. The modular bioreactors are simple to assemble and use and can be easily compared with standard controls since cell numbers and medium volumes are quite similar. Starting from fluid dynamic and mass transport considerations, a modular bioreactor chamber was first modeled and then fabricated using "milli-molding," a technique adapted from soft lithography. After confirming that the shear stress was extremely low in the system in the range of useful flow rates, the bioreactor chambers were tested using hepatocytes. The results show that the bioreactor chambers can increase or maintain cell viability and function when the flow rates are below 500 microL/min, corresponding to wall shear stresses of 10(-5) Pa or less at the cell culture surface.</p>
10aBioengineering10aBioreactors1 aMazzei, D.1 aGuzzardi, M. A.1 aGiusti, S1 aAhluwalia, A.  uhttp://615830.rige.asia/publications/low-shear-stress-modular-bioreactor-connected-cell-culture-under-high-flow-rates.html